Immunological Study of Juvenile Hormone Binding Protein from Hemolymph of the Fall Webworm (Lepidoptera: Arctiidae)
The juvenile hormone binding protein (JHBP) was purified from the hemolymph of Hyphantria cunea (Lepidoptera: Arctiidae) using anion exchange, gel filtration, and Mono P FPLC chromatofocusing chromatography. The protein is a single polypeptide (Mr 32,000) with an apparent dissociation constant of 0.43 M for juvenile hormone III. An antibody developed against hemolymph JHBP (hJHBP) was prepared, and use of it showed that a protein immunologically identical with hJHBP occurs in fat body and ovary. The hJHBP of H. cunea was neither immunologically related to the lipophorin from H. cunea nor similar to the hJHBP of Bombyx mori and Periplaneta americana.