Comparing effects of three acaricides on Varroa jacobsoni (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae) using two application techniques.

Gerardo Prez Santiago, Gabriel Otero-Colina, David Mota Snchez, Martha Elva Ramirez Guzman, Remy Vandame


Two bioassays were administered to determine the dose-lethality response of Varroa jacobsoni Oudemans and the honey bee, Apis mellifera L., to amitraz, flumethrin and fluvalinate. The first bioassay method was spraying by means of the Potter-Bourgerjon's tower. The results are expressed in mean lethal concentrations (LC50). The second method was topical application by means of microsyringe and manual applicator. The results are expressed in mean lethal doses (LD50). Both LC50 and LD50 values were considerably higher in honey bees than in varroa mites, showing that a wide margin of safety exists between effective doses against mites and harmful doses for honey bees. Both methods gave similar confidence intervals; they showed a comparable sensitivity to changes in dose or concentration of pesticides.

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