Timing of irradiation and male mating history effects on female remating in Anastrepha ludens (Diptera: Tephritidae)

José Arredondo, Marco T Tejeda, Lia Ruiz, José S. Meza, Diana Pérez-Staples


Abstract For the sterile insect technique to be successful, sterile males need to mate with wild females, transfer an ejaculate and inhibit females from remating. This study evaluated the capacity of irradiated Anastrepha ludens (Loew) (Diptera: Tephritidae) males of the genetic sexing strain Tapachula 7 (Tap-7) for inducing the sexual refractory period (time between the first and second mating) of wild females. The following factors were evaluated: 1) irradiation versus no irradiation of male pupae, 2) the age at which male pupae were irradiated (72, 48, and 24 h before adult emergence), and 3) male sexual experience (virgin, once mated, and twice mated). There was no effect of irradiation or age at irradiation on male ability to inhibit female remating or on sexual refractory period. However, wild females had shorter refractory periods when mated with virgin Tap-7 males, compared with males that previously had mated once or twice. We suggest that because inhibition of remating in A. ludens is determined by the full male ejaculate, virgin Tap-7 males are probably delivering smaller ejaculates compared with sexually experienced males. This study increases our knowledge of the reproductive biology of the genetic sexing strain of A. ludens.


Resumen Para que la técnica del insecto estéril sea exitosa, los machos estériles necesitan aparearse con hembras silvestres, transferir un eyaculado e inhibir el re-apareamiento de las hembras. Este estudio evaluó la capacidad de machos irradiados de Anastrepha ludens (Loew) (Diptera: Tephritidae) de la cepa sexada genéticamente Tapachula 7 (Tap-7) para inducir el periodo refractorio sexual (tiempo transcurrido entre la primera y segunda cópula) de hembras silvestres de campo. Los siguientes factores fueron evaluados: 1) irradiados versus no irradiados, 2) la edad a la que la pupa fue irradiada (72, 48, y 24 h antes de la emergencia), y 3) la experiencia sexual del macho (virgen, 1 cópula, o 2 cópulas). La irradiación o la edad a la que la pupa fue irradiada no tuvo efecto sobre la habilidad de los machos para inhibir el re-aparamiento de la hembra o sobre su periodo refractorio sexual. No obstante, las hembras de campo presentaron periodos refractorios menores cuando se aparearon con machos vírgenes de la cepa Tap-7, al compararse con machos que previamente se aparearon una o dos veces. Se sugiere que, dado que la inhibición del re-apareamiento en A. ludens está determinada por todos los componentes del eyaculado, los machos vírgenes de la cepa Tap-7 probablemente transfieren eyaculados más pequeños que los transmitidos por machos con experiencia sexual. Este estudio aumenta nuestro conocimiento sobre la biología reproductiva de la cepa sexada genéticamente de A. ludens.


View this article in BioOne


copulation; mass production; SIT; sterility;

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