Genetic Variation in Sucrose Metabolizing Enzymes among Six Muscadine Varieties

Devaiah Kambiranda, Hemanth Kn. Vasanthaiah, Sheikh M. Basha

Abstract


Accumulation of sugars is an important process that occurs during grape berry development. Sugars are transported into the berry mainly in the form of sucrose and are broken down into glucose and fructose by the enzymes invertase and sucrose synthase. In this study we have determined changes in the soluble sugar content, and invertase and sucrose synthase enzyme activity levels during different stages (30, 60, 90 d, and ripe) of berry development among selected muscadine varieties. Soluble sugar content varied among muscadine genotypes, with the highest being in ‘African Queen’ and lowest in ‘Carlos’. Expression of invertase enzyme was highest in 90-d-old berry as well as in ripened berry among the muscadine varieties tested. Sucrose synthase activity was highest in 90-d-old berries among ‘Summit’, ‘Scarlet’, and ‘African Queen’ cultivars. In contrast, ‘Noble’, ‘Carlos’, and ‘Welder’ contained the highest sucrose synthase activity in 60-d-old berries. These data suggest that in muscadine grape berry invertase and sucrose synthase activities are positively correlated with high berry sugar content.

Keywords


Muscadinia rotundifolia, sugars, invertase, sucrose synthase

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