Development of mapping populations for avocado

Cecile Olano, James Borrone, J. Steven Brown, Helen Violi, Randy Ploetz, Raymond Schnell

Abstract


A saturated genetic linkage map can aid greatly in efforts to improve avocado (Persea americana Mill.) via breeding using a Marker Assisted Selection (MAS) approach. A genetic linkage map for avocado can be generated using molecular genetic markers and a known family structure. Currently, approximately 300 microsatellite markers have been developed for avocado. This number of microsatellite markers should give suitable coverage for the avocado genome. Two experimental families have been identified: a Florida mapping population [West Indian x (Guatemalan x West Indian)] of 1926 seeds harvested from a commercial grove in south Miami-Dade County, FL, and a California mapping population [Mexican x (Guatemalan x Mexican)] of 2030 seeds harvested from a commercial grove in Ventura County, CA. Phenotypic evaluation of the mapping population will allow the identification of Quantitative Trait Loci (QTL) that are associated with important horticultural traits including productivity, fruit quality and cold tolerance. The production of a saturated linkage map will lead to a better understanding of the avocado genome. QTL will be used in a MAS program that is being established.

Keywords


molecular breeding; Persea americana; linkage mapping

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Proc. Fla. State Hort. Soc.     ISSN 0886-7283