Investigation of the origin of 'Champneys' pink cluster', 'Blush noisette' and 'napoleon' roses using randomly amplified polymorphic DNA (rapd) analysis

Amanda Wagner, Carmella Frederick, Nancy Morvillo

Abstract


Utilizing randomly amplified polymorphic DNA (RAPD) analysis, the parentage of accessions of 'Champneys' Pink Cluster' and 'Blush Noisette' from several nursery sources was investigated. Nearly all polymorphic DNA banding patterns in the profile of the accessions of 'Champneys' Pink Cluster' were accounted for in 'Old Blush' (Rosa chinensis Jacquin hybrid) and the musk varieties (Rosa moschata Herrmann), and many bands in the 'Blush Noisette' accessions were accounted for in 'Old Blush' and the musks. This confirms the parentage of these accessions. 'Old Blush' was also compared to 'Napoleon' via RAPD analysis to investigate the genetic similarities between them. The profiles showed almost identical banding patterns. This suggests the two species are closely related, consistent with reports that 'Napoleon' is a triploid of 'Old Blush'.

Keywords


hybrid; parentage; pcr; rapd; rose genomic dna; triploid

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Proc. Fla. State Hort. Soc.     ISSN 0886-7283