Micropropagation of Smooth Flat Seville and Yuma

Kim D. Bowman


Smooth Flat Seville (Citrus spp. hybrid of uncertain origin) and Yuma {Poncirus trifoliata [L.] Raf. x Citrus sp. hybrid of uncertain origin) are cultivars that have exhibited good citrus rootstock characteristics in some field tests. However, both of these selections are difficult to propagate commercially as rootstocks from seed because they produce an unacceptably large percentage of zygotic seedlings. The objective of this research was to define efficient tissue culture propagation protocols that could allow the large scale generation of uni form plantlets from each of these cultivars. Media and proce dures have been defined for entering plant material into sterile culture, producing multiple shoots from single node explants, and rooting shoot segments in culture. Successful procedures for micropropagation of these cultivars will be described.

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Proc. Fla. State Hort. Soc.     ISSN 0886-7283