A Comparison of Pectinases from Ditylenchus dipsaci and Allium cepa Callus Tissue

R. M. Riedel, W. F. Mai

Abstract


Ground and whole Ditylenchus dipsaci maintained on onion callus contain no culturable micro-organisms when tested with five check media. Healthy onion callus does not produce pectolytic enzymes. Pectolytic enzymes are present in infected callus. These enzymes are, however, associated with resident nematodes and not host tissues. These results suggest that D. dipsaci is the actual source of the endo-polygalacturonase and endo-pectinmethyltrans-eliminase extracted from them. Key Words: Enzyme, Onion.

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