Biochemical Identification of the Two Races of Radopholus similis by Starch Gel Electrophoresis

R. N. Huettel, D. W. Dickson, D. T. Kaplan

Abstract


Analysis of genetic variation between the banana and the citrus races of Hadopholus similis by starch gel eleclrophoresis demonstrated that 7 of 16 enzyme-encoding loci could be used for their diagnostic separation. The two races are closely related arid share approximately 75% of the enzymes evaluated. The level of dissimilarities o1 inherited bands indicates that no gene flow occurs between the races. Aldolase,[alpha] +[beta] esterase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, lactate dehydrogenase, malate dehydrogenase, and phosphoglucose isomerase are diagnostic markers of the races. Key words: isozyme, nematode races, burrowing nematode, genetics, biosystematics.

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