Application of Isoelectric Focusing to the Taxonomic Identification of Meloidogyne spp.

Edgar C. Lawson, III, George E. Carter, Jr., Stephen A. Lewis

Abstract


Meloidogyne incognita, M. arenaria, M. hapla, and M. javanica were distinguishable from each other by isoelectric focusing (IEF) of nematode egg proteins. Proteins extracted from larvae and adults of Hoplolaimus columbus and from eggs of Heterodera glycines had distinctive profiles, also. Protein profiles from eggs, preparasitic larvae and egg-laying adults of M. incognita showed differences. It was necessary to compare samples run at the same time to ensure reliability. Key words: root-knot nematodes, biochemical taxonomy, isoelectric focusing, MeloidogTne spp., protein profiles.

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