DNA Complexity of the Root-knot Nematode (Meloidogyne spp.) Genome

E. C. Pableo, A. C. Triantaphyllou


Cot curves derived from renaturation kinetics of sheared denatured DNA indicated that the genome of six populations representing the four most common root-knot nematode species (Meloidogyne incoffnita, M. arenaria, M. javanica, and M. hapla) is composed of 20% repetitive and 80% nonrepetitive sequences of DNA. Cot curves were almost identical, indicating that all populations had a haploid genome of approximately the same size. Calculations from an average Cot curve gave an estimate of 0.51 x 108 nucleotide base pairs for the haploid genome of the four Meloidogyne species. This genome is about 12-13 times larger than the genome of the E. coli strain used as a control. Key words: Cot analysis, DNA, Meloidogyne, repetitive DNA, root-knot nematode, unique DNA.

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