Comparisons of Isozyme Phenotypes in Five Meloidogyne spp. with Isoelectric Focusing

Sudha Venkatachari, L. A. Payan, D. W. Dickson, T. E. Hewlett

Abstract


Meloidogyne incognita race 1, M. javanica, M. arenaria race 1, M. hapla, and an undescribed Meloidogyne sp. were analyzed by comparing isozyme phenotypes of esterase, malate dehydrogenase, phosphoglucomutase, isocitrate dehydrogenase, and[alpha]-glycerophosphate dehydrogenase. Isozyme phenotypes were obtained from single mature females by isoelectric focusing electrophoresis. Of these five isozymes, only esterase and phosphoglucomutase could be used to separate all five Meloidogyne spp.; however, the single esterase electromorphs were similar for M. incognita and M. hapla. Yet when both nematodes were run on the same gel, differences in their esterase phenotypes were detectable. Isozyme phenotypes from the other three isozymes revealed a great deal of similarity among M. incognita, M. javanica, M. arenaria, and the undescribed Meloidogyne sp. Key words: biochemical systematics, electrophoresis, enzyme, esterase,[alpha]-glycerophosphate dehydrogenase, isocitrate dehydrogenase, isoelectric focusing, malate dehydrogenase, Meloidogyne arenaria, M. hapla, M. incognita, M. javanica, nematode, phosphoglucomutase.

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