Comparison of Assays for the Determination of Entomogenous Nematode Infectivity

Steven R. Sims, Amy S. Downing, Jay C. Pershing

Abstract


Injection, contact, and soil assays were used to compare infectivity of Heterorhabditis bacteriophora strain HP88 and Steinernema carpocapsae strain All to final instar Galleria mellonella larvae. Under comparable assay conditions, H. bacteriophora produced less Galleria mortality and showed greater within-assay variability in infectivity than S. carpocapsae. Injection of individual S. carpocapsae or H. bacteriophora infective juveniles into Galleria indicated that a comparatively greater percentage of S. carpocapsae was capable of initiating infection. In addition to nematode species, other major components of variability in assay estimations of nematode infectivity were number of nematodes used in the assay, assay type, date of the assay, and possibly, Galleria age. Key words: Bioassay, infectivity assay, injection, nematode, Galleria mellonella, Heterorhabditis bacteriophora, Steinernema carpocapsae.

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