Characterization of Anionic Peroxidases in Tomato Isolines Infected by Meloidogyne incognita

G. Zacheo, C. Orlando, T. Bleve-Zacheo

Abstract


Changes in peroxidase activity during nematode infection were studied using root extracts of tomato near-isogenic lines differing in resistance to Meloidogyne incognita. Total peroxidase activity increased slightly in crude extracts of four susceptible isolines but doubled in two resistant lines, Monita and Motaci. Nematode infection enhanced levels of both p-phenylenediamine-pyrocatechol oxidase and syringaldazine oxidase 7 days after inoculation, especially in resistant lines. This elevated peroxidase activity in resistant isolines was caused by an increase in anionic peroxidase activity. These enzymes, which likely are involved in lignification, were isolated and purified from tomato isolines by ammonium sulfate precipitation, high performance ion-exchange chromatography, and gel electrophoresis. The purified anionic peroxidase extracts contained an electrophoretic band with R[subf] 0.51 that was present in extracts of infected but not uninfected roots. Key words: enzyme, isoline, isoperoxidase, Lycopersicon esculentum, Meloidogyne incognita, nematode, peroxidase, resistance.

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