Effect of Temperature on Pratylenchus penetrans Development

Takayuki Mizukubo, Hiroshi Adachi

Abstract


Reproduction and development of Pratylenchus penetrans were studied on genetically transformed ladino clover roots. Solitary females developing on transformed roots in nutrient gellan gum medium (pH 5.5) deposited 1.2, 1.5, 1.6, 1.8, and 2.0 eggs per day at the respective temperatures of 17, 20, 25, 27, and 30ºC. The number of eggs deposited was highly correlated with temperature. A reduction in egg-laying rates at the start of hatching was observed at all temperatures. Juvenile mortality was higher at 17ºC (50.4%), 20ºC (50.3%), and 30ºC (58.4%) than at 25ºC (34.6%) and 27ºC (37.6%). Life-cycle (egg deposition to egg deposition) duration was 46, 38, 28, 26, and 22 days at the respective temperatures. The developmental zero degrees (ºC) and the effective accumulative temperatures (degree-days) required for hatching, female emergence, and onset of oviposition (completion of one generation) of P. penetrans were estimated to be 2.7 and 200, 4.2 and 548, and 5.1 and 564, respectively. Pratylenchus penetrans reproduces over a wide range of temperatures. Key words: degree-day, developmental zero degree, egg-laying rate, effective accumulative temperature, ladino clover, life cycle, nematode, Pratylenchus penetrans, reproduction, transformed hairy root.

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