A Method for Isolation of Pasteuria penetrans Endospores for Bioassay and Genomic Studies

Jenora T. Waterman, David McK. Bird, Charles H. Opperman

Abstract


A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations.

Keywords


endospores; method; Pasteuria penetrans.

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