Ribosomal and Mitochondrial DNA Analyses of Xiphinema americanum -Group Populations

Stela S. Lazarova, Gaynor Malloch, Claudio M. G. Oliveira, Judith Hubschen, Roy Neilson


The 18S ribosomal DNA (rDNA) and cytochrome oxidase I region of mitochondrial DNA (mtDNA) were sequenced for 24 Xiphinema americanum-group populations sourced from a number of geographically disparate locations. Sequences were subjected to phylogenetic analysis and compared. 18S rDNA strongly suggested that only X. pachtaicum, X. simile (two populations) and a X. americanum s.l. population from Portugal were different from the other 20 populations studied, whereas mtDNA indicated some heterogeneity between populations. Phylogenetically, based on mtDNA, an apparent dichotomy existed amongst X. americanumgroup populations from North America and those from Asia, South America and Oceania. Analyses of 18S rDNA and mtDNA sequences underpin the classical taxonomic issues of the X. americanum-group and cast doubt on the degree of speciation within the X. americanum-group.


18S rDNA; longidorid; Longidoridae; molecular analysis; mtDNA; nematode; taxonomy.

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