Isolation of a Repeated DNA Probe Showing Polymorphism among Meloidogyne incognita Populations

P. Castagnone-Sereno, C. Piotte, P. Abad, M. Bongiovanni, A. Dalmasso

Abstract


Several Meloidogyne incognita geographic populations were characterized by analysis of the restriction fragment length polymorphisms (RFLP) obtained after digestion of their total DNA and hybridization with a [32P]-labeled probe. The probe consisted of a 1.7-kb-repeated DNA sequence, isolated from a M. incognita genomic library, that hybridized to multiple BamH I fragments in the genome of each isolate. The patterns showed sufficient polymorphism to enable the accurate differentiation of all the populations tested. Key words: DNA, hybridization, intraspecific differentiation, Meloidogyne incognita, repetitive DNA.

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