A FRAGMENT OF THE LARGE SUBUNIT OF THE rRNA GENE AMPLIFIED BY POLYMERASE CHAIN REACTION IN INDIVIDUAL NEMATODES

C. De Giorgi, M. Fineti Sialer, M. Di Vito, F. Lamberti

Abstract


The Polymerase Chain Reaction (PCR) technique has been used for the amplification of a small portion at the 5' end of the large subunit of rRNA gene in different genera of plant parasitic nematodes. Furthermore, by using several different species of Xiphinema, a technique has been developed by which the amplification of the same fragments in a single individual is achieved. Studies on the amplified fragments strongly suggest that their sequences and their organization in the genome are species-specific.

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