IN VITRO NEMATICIDAL ACTIVITY OF EXTRACTS OF BULBS AND SEEDS OF ONION AGAINST ROOT-KNOT NEMATODE MELOIDOGYNE INCOGNITA

E. S. J. Nidiry, M. V. Chandravadana, R. M. Khan, M. S. Rao

Abstract


Among the various solvent extracts of the bulbs and seeds of onion assayed far in vitro nematicidal activity against root-knot nematode, Meloidogyne incognita, hot methanol extract of defatted seeds exhibited significant activity. The active extract contained a compound which fluoresced in ultra violet light; it coloured pink when sprayed with diazotized sulphanilic acid followed by sodium hydroxide on a TLC plate. Free amino acids viz. leucine (isoleucine), aspartic acid, methionine and threonine each exhibited nematicidal activity, but to a much lesser extent than the extract itself.

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