Biometric and molecular characterization and juvenile development stages of Xiphinema vulgare Tarjan, 1964 (Nematoda, Dorylaimida)

F. Lamberti, L. W. Duncan, F. De Luca, S. Molinari, A. Agostinelli, D. Dunn, M. I. Coiro, V. Radicci

Abstract


Morphometric and molecular studies were undertaken of Floridian populations of Xiphinema vulgare Tarjan, 1964. All populations were found to be almost morphometrically identical with the type populations and those from Sao Tome. Juveniles were clearly separated in four developmental stages. Isoelectricfocusing profiles of specimen extracts stained for superoxide dismutase activity showed one or two close bands at pH 7.6 and 7.6/7.3, respectively. The peR products of the ITS region was between 1 and 1.5 kb and was digested by six restriction enzymes.

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