Research Papers: Effect of Sieve Size on Nematode Extraction Efficiency

McSorley, R.; Parrado, J. L.

Abstract


A combination sieving and centrifugation procedure was used to compare the efficiency of recovering nematodes from soil suspensions using sieves having openings of various sizes: 53 m, 45 m, 38 m, or 25 m. With the 53 m, 45 m, and 38 m sieve sizes, there were no differences in numbers extracted for larger nematodes such as Helicotylenchus dihystera or Criconemella spp., but the 38 m sieve was the most efficient in recovering larvae of Rotylenchulus reniformis. Because the openings of the 25 m sieve became clogged with soil particles, nematode recovery was poor from that sieve size for the Rockdale soil type tested here. The effect of decanting nematode suspensions in sucrose solutions onto sieves having 45 m or 25 m openings was also examined. In this case, recovery of H. dihystera was unaffected by sieve size, but the 25 m sieve was more suitable for recovering R. reniformis larvae. In two tests, rinsing the nematodes on the sieve to remove the sugar solution resulted in a significa

Keywords


Reniform Nematode; Spiral Nematode; Soil Type; Carica Papaya; Ipomoea batatas; Manihot Esculenta; Phaseolus aureus

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