Research Papers: A Simple Incubation Method for the Extraction of Nematodes from Soil

Rodriguez-Kabana, R.; Pope, M. H.

Abstract


A simple method for the extraction of nematods from soil was developed using commonly available materials. With the method 100 cm soil were spread on 2-ply "Scottie" tissue paper on a sieve consisting of fiberglass screen glued between two PVC pipe sections. The sieve with the soil was placed in a plastic bowl and water was added to just cover the soil. The sieve with soil was incubated for 72 h when nematodes in the water were collected by passing the water through a 38 m screen. Comparisons between this method and a flotation sieving technique indicated that numbers of nematodes extracted by the two techniques were significantly correlated. The bowl method resulted in extraction of higher numbers of Pratylenchus Filipjev larvae of Heterodera glycines Ichinohe and Meloidogyne Goeldi but less numbers of Helicotylenchus Steiner and Hoplolaimus Daday than the flotation technique.

Keywords


Methodology; Diagnostic Services; Root-Knot Nematodes; Soybean Cyst Nematodes; Spiral Nematodes; Lance Nematodes; Lesion Nematodes

Full Text: PDF