EXCISED ROOT CULTURE FOR MASS PRODUCTION OF HOPLOLAIMUS COLUMBUS SHER (NEMATA: TYLENCHIDA)
Experiments with a monoxenic culture of Hoplolaimus columbuson excised roots were conducted to evaluate effects of temperature and initial population density (Pi) on final population numbers (Pf), evaluate host range, and compare virulence and host specificity to that in field populations. The nematodes fed and reproduced on excised root cultures, with average reproductive factors (Pf/Pi) of 254 on alfalfa and 121 on soybean after 90 days. Incubation at 30ºC and an initial population of 10 nematodes per 9-cm petri dish were optimal for reproduction. Nematodes maintained in excised root culture for one year retained their virulence and host specificity in the greenhouse when compared to extracted field populations.
alfalfa; culture; excised root; hoplolaimus columbus; host; lance nematode; medicago sativa; reproductive factor; soybean; virulence